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Research Topics
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 Research Topics    |    Major Focus Areas
Research Topics
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Lung & Respiratory
Project Summary

Title: Surveillance of B. pertussis among Military Trainees with Respiratory Disease: Development and Validation of a Highly Sensitive PCR and Beacon Probe based Method for Diagnosis of Pertussis
Synopsis: The goal of this study is to develop a standardized method for rapidly detecting respiratory disease (B pertussis) among military trainees.
Overall Project Objective: Develop and standardize a sensitive and reliable detection method for effective surveillance of B. pertussis infection among military trainees with respiratory disease. Determine whether or not B. pertussis infection is one of the major causes of respiratory disease-related morbidity among military recruits.
Status/Results to Date: A sensitive beacon probe has been developed and is being used to assess the prevalence of pertussis infection among young adults with respiratory illness at several basic training centers.
Project:DoD-97
Agency:Department Of Defense
Location:Naval Health Research Center
P.I. Name:Margaret A.K. Ryan, M.D., LCDR
Research Type:Development
Research Focus:Diagnosis
Focus Category:Lung & Respiratory
Status:Ongoing
Study Start Date:October 01,1999
Estimated Completion Date:September 30,2002
Specific Aims: Establish a reliable and rapid method for pertussis evaluation in military trainees and determine contribution of pertussis infection to respiratory disease in a trial group of military trainees.
Methodology: A rapid diagnostic method based on Polymerase Chain Reaction (PCR) and molecular beacon probe technology will be standardized and developed for B pertussis detection. This PCR method along with two standard methods (Culture and Serology) will be applied to assess the prevalence of B pertussis in the above mentioned population suffering from 7 or more days of coughing and other respiratory problems.
Most Recent Publications:

Le CT, Gray G, Poddar SK. A modified rapid method of nucleic acid isolation from suspension of matured virus: applied in restriction analysis of DNA from an adenovirus prototype strain and a patient isolate. J Med Microbiol, 50(6):571-4, Jun 2001. Abstract

Poddar SK, Le CT. Bordetella pertussis detection by spectrofluorometry using polymerase chain reaction (PCR) and a molecular beacon probe. Mol Cell Probes, 15(3):161-7, Jun 2001. Abstract

Fuller J, McKeehan J, Hudspeth M, Malasig M, Gray G, Schultz R, Gackstetter GD, Thomas R. Etiology and Incidence of Respiratory Disease Infections at the US Naval Academy. 40th Navy Occupational and Preventive Medicine Workshop, Norfolk, VA, 29 Jan-4 Feb, 2000. Article